Microsatellite markers for plants of the species triticum aestivum and tribe triticeae and the use of said markers

ABSTRACT

The invention pertains to novel genetic markers for wheats (Triticum aestivumL.) and closely related species (tribe: Triticeae) and to the use of these markers. The aim of the invention is to develop novel microsatellite markers intended for use in the genetic analysis of plants of the species Triticum aestivum and characterised by a higher level of DNA polymorphism than other molecular probes hitherto developed for the wheat genome. The invention involves the preparation of 233 microsatellite markers, or a proportion thereof, which are defined as primer pairs with associated microsatellite sequences and amplify loci of various chromosomes of the wheat genome and are therefore suitable for use in genetic marking.

[0001] The invention relates to novel genetic markers for wheats(Triticum aestivum L.) and closely related species (Tribus Triticeae)and to the use of said markers.

[0002] The most widely spread, known, DNA-based genetic markers are theso-called restriction fragment length polymorphisms (RFLP) markers. Forusing these markers, genomic DNA is digested with restriction enzymes,separated on agarose gels and transferred to nylon membranes (SouthernBlot). Specific fragments are detected by hybridization withradioactively labeled DNA probes. When mutations occur in the region ofthe restriction enzymes used or when smaller deletions/insertions occur,polymorphisms between different lines are found, which are passed onstably and mostly codominantly. The use of RFLP markers in hexaploidcultivated wheat is possible only to a limited extent, since only verylittle polymorphism is detected in wheat in this manner.

[0003] It has already been described that microsatellite markers detectsignificantly more polymorphism between different wheat lines than doRFLP markers. This can be attributed particularly to the occurrence ofmultiple alleles per locus (Rbder et al., Mol. Gen. Genet. (1995) 246,327 - 333). Moreover, it is known that microsatellite markers have theadvantage that they can be detected by way of PCR and that thereforelarge amounts of samples can be analyzed more easily.

[0004] It is an object of the invention to provide novel microsatellitemarkers for the genetic analysis of plants of the Triticum aestivumspecies, which markers are distinguished by a degree of DNApolymorphism, which is higher than that of other molecular probes, thathave been developed previously for the wheat genome.

[0005] This objective is accomplished by claims 1 to 10. The inventivemarkers are based on the amplification of certain hypervariable genomesections, the so-called microsatellites, with the help of theirpolymerase chain reaction (PCR). For specific amplification, twoprimers, in each to the case left and the right in the flankingsequences, are required for each microsatellite locus. On the average,these primers are 20 i 3 bases long and are defined by their sequences.In principle, a microsatellite marker is a sequence tagged site (STS),which is defined by two specific primers. These primers flank, in eachcase to the left and the right, a so-called microsatellite sequence. Amicrosatellite sequence is defined as a tandem repetitive repetition ofa di-, tri- or tetranucleotide sequence, for example (GA)_(n), in whichn>10. Composite microsatellite sequences also occur, such as(GT)_(n)(AT)_(n), as well as imperfect sequences, in which individualbases are mutated, such as (GA)_(n)CA(GA)_(n). Among various lines andvarieties, there is variation in the number of repeats at a certainlocus. After amplification of the microsatellites, this leads, by meansof the specific primers in the flanking sequences, to PCR products ofdifferent length and, with that, to polymorphisms. These polymorphismsare passed on stably and can therefore be used as genetic markers. Insome cases, null alleles (no visible fragment) also occur, when thereare mutations within the binding site for the primers.

[0006] The separation and detection of the PCR products obtained can becarried out with different technical variants. For separating thefragments, highly resolving agarose gels, native polyacrylamide gels ordenaturing polyacrylamide gels (=sequencing gels) can be used. Dependingon the separation system, fragments are detected using ethidium bromidestaining, silver staining or, after labeling the PCR fragmentsradioactively, using autoradiography. A further, very effectivevariation for separation and detection consists of the use of anautomatic sequencer with dye- or fluorescence-labeled primers. For thispurpose, it is necessary to synthesize a dye- or fluorescence-labeledprimer from each microsatellite primer pair. PCR amplification resultsin a labeled product, which can be detected by the sequencing equipment.At the same time, dye- or fluorescence-labeled size standards are alsoseparated for each sample in the same track. After that, specialsoftware enable the absolute size of each fragment, which has beenseparated, to be calculated and, with that, also permits fragments fromdifferent gel runs to be compared. With this method, several hundredsamples can be analyzed largely automatically in a day.

[0007] Pursuant to the invention, microsatellite markers are madeavailable, which contain the following primer pairs with assignedmicrosatellite sequences or a number thereof and amplify the loci of allchromosomes of the wheat genome and therefore find use for gene marking.Anneal- ing Length Temper- (bp in ature WMS “cs”) Anneal- Number Längeing- WMS- WMS Primer Left WMS Primer Right (bp) in Repeat Type Temper-Number WMS Primer Links WMS Primer rechts “CS” Repeat-Typ atur WMS052 5′CTA TGA GGC GGA GGT TGA AG 3′ 5′ TGC GGT GCT CTT CCA TTT 3′ 150 GTimp60° C. WMS055 5′ GCA TCT GGT ACA CTA GCT GCC 3′ 5′ TCA TGG ATG CAT CACATC CT 3 127 GTimp 60° C. WMS057 5′ TCG ATT CTG AAA GGT TCA TCG 3′ 5′CGA TCA AGT AGT TGA AAG CGC 3′ 224 AAAAAimp 60° C. WMS058 5′ TCT GAT CCCGTG AGT GTA ACA 3′ 5′ GAA AAA AAT TGC ATA TGA GCC 118 CA 60° C. C 3′WMS060 5′ TGT CCT ACA CGG ACC ACG T3′ 5′ GCA TTG ACA GAT GCA CAC G 3′211 CA 60° C. WMS063 5′ TCG ACC TGA TCG CCC CTA 3′ 5′ CGC CCT GGG TGATGA ATA GT 3′ 271 GAA,CA,TA 60° C. WMS067 5′ ACC ACA CAA ACA AGG TAA GCG3′ 5′ CAA CCC TCT TAA TTT TGT TGG 85 CA 60° C. G 3′ WMS068 5′ AGG CCAGAA TCT GGG AAT G 3′ 5′ CTC CCT AGA TGG GAG AAG GG 3′ 182 GA 60° C.WMS070 5′ AGT GGC TGG GAG AGT GTC AT 3′ 5′ GCC CAT TAC CGA GGA CAC 3′194 GT 60° C. WMS071 5′ GGC AGA GCA GCG AGA CTC 3′ 5′ CAA GTG GAG CATTAG GTA CAC 128 GT 60° C. G 3′ WMS077 5′ ACA AAG GTA AGC AGC ACC TG 3′5′ ACC CTC TTG CCC GTG TTG 3′ 153 CA,GA 55° C. WMS082 5′ ACG TTA GAA GGTGCA ATG GG 3′ 5′ AGT GGA TGC ACC GAC TTT G 3′ 152 GT,GAimp 60° C. WMS0885′ CAC TAC AAC TAT GCG CTC GC 3′ 5′ TCC ATT GGC TTC TCT CTC AA 3′ 121 GT60° C. WMS095 5′ GAT CAA ACA CAC ACC CCT CC 3′ 5′ AAT GCA AAG TGA AAAACC CG 3′ 121 CA 60° C. WMS099 5′ AAG ATG GAC GTA TGC ATC ACA 3′ 5′ GCCATA TTT GAT GAC GCA TA 3′ 119 CA 60° C. WMS102 5′ TCT CCC ATC CAA CGCCTC 3′ 5′ TGT TGG TGG CTT GAC TAT TG 3′ 143 CT 60° C. WMS106 5′ CTG TTCTTG CGT GGC ATT AA 3′ 5′ AAT AAG GAC ACA ATT GGG ATG 139 GA 60° C.WMS107 5′ ATT AAT ACC TGA GGG AGG TGC 3′ 5′ GGT CTC AGG AGC AAG AAC AC3′ 195 CT 60° C. WMS108 5′ CGA CAA TGG GGT CCT AGC AT 3′ 5′ TGC ACA CTTAAA TTA CAT CCG 132 GTimp 60° C. C 3′ WMS111 5′ TCT GTA GGC TCT CTC CGACTG 3′ 5′ ACC TGA TCA GAT CCC ACT CG 3′ 205 CT,GT 55° C. WMS112 5′ CTAAAC ACG ACA GCG GTG G 3′ 5′ GAT ATG TGA GCA GCG GTC AG 3′ 101 CTimp 55°C. WMS113 5′ ATT CGA GGT TAG GAG GAA GAG G 3′ 5′ GAG GGT GGG CCT ATA AGACC 3′ 148 GT 60° C. WMS114 5′ ACA AAC AGA AAA TCA AAA CCC G 3′ 5′ ATCCAT CGC CAT TGG AGT G 3′ 206 GA 60° C. (177) WMS118 5′ GAT GTT GCC ACTTGA GCA TG 3′ 5′ GAT TAG TCA AAT GGA ACA CCC 110 CA 60° C. C 3′ WMS1195′ TGA CTA ACA TCC TTT GTC ACG C 3′ 5′ CAT GTC TCA ACC ACC CAC AG 3′ 181GTimp 55° C. WMS120 5′ GAT CCA CCT TCC TCT CTC TC 3′ 5′ GAT TAT ACT GGTGCC GAA AC 3′ 139 CT,CA 55° C. WMS121 5′ TCC TCT ACA AAC AAA CAC AC 3′5′ CTC GCA ACT AGA GGT GTA TG 3′ 143 CA 50°C WMS122 5′ GGG TGG GAG AAAGGA GAT G 3′ 5′ AAA CCA TCC TCC ATC CTG G 3′ 149 CT,CA 60° C. WMS124 5′GCC ATG GCT ATC ACC CAG 3′ 5′ ACT GTT CGG TGC AAT TTG AG 3′ 213 CT,GTimp60° C. WMS126 5′ CAC ACG CTC CAC CAT GAC 3′ 5′ GTT GAG TTG ATG CGG GAG G3′ 196 CA 60° C. WMS128 5′ AGC ACA TTT TAA CAC AGA TA 3′ 5′ ATC TGT GAAATT TTG AAA AC 3′ 176 CA 50°C WMS129 5′ TCA GTG GGC AAG CTA CAC AG 3′ 5′AAA ACT TAG TAG CCG CGT 3′ 221 GTimp 55° C. WMS130 5′ AGC TCT GCT TCACGA GGA AG 3′ 5′ CTC CTC TTT ATA TCG CGT CCC 3′ 113 GT 60° C. WMS131 5′AAT CCC CAC CGA TTC TTC TC 3′ 5′ AGT TCG TGG GTC TCT GAT GG 3′ 131 CT60° C. WMS132 5′ TAC CAA ATC GAA ACA CAT CAG G 3′ 5′ CAT ATC AAG GTC TCCTTC CCC 3′ 119 GA,GAA 60° C. WMS133 5′ ATC TAA ACA AGA CGG CGG TG 3′ 5′ATC TGT GAC AAC CGG TGA GA 3′ 118 CT 60° C. WMS134 5′ CAT GGA ACT TAGACA GAA TTG 3′ 5′ CAG TAC TTG GTA CTG AAC AGG 3′ 111 CA 60° C. WMS135 5′TGT CAA CAT CGT TTT GAA AAG G 3′ 5′ ACA CTG TCA ACC TGG CAA TG 3′ 143 GA55° C. WMS136 5′ GAC AGC ACC TTG CCC TTT G 3′ 5′ CAT CGG CAA CAT GCT CATC 3′ 296 CT 60° C. WMS140 5′ ATG GAG ATA TTT GGC CTA CAA C 3′ 5′ CTT GACTTC AAG GCG TGA CA 3′ 251 CT 55° C. WMS144 5′ TTT GCT GTG GTA CGA AACATA C 3′ 5′ ACT CAC AAA TGT CTA ATA AAA 200 GT 50°C C 3′ WMS146 5′ CCAAAA AAA CTG CCT GCA TG 3′ 5′ CTC TGG CAT TGC TCC TTG G 3′ 162 GAimp 60°C. WMS148 5′ GTG AGG CAG CAA GAG AGA AA 3′ 5′ CAA AGC TTG ACT CAG ACCAAA 3′ 163 CA 60° C. WMS149 5′ CAT TGT TTT CTG CCT CTA GCC 3′ 5′ CTA GCATCG AAC CTG AAC AAG 3′ 161 GA 55° C. WMS153 5′ GAT CTC GTC ACC CGG AATTC 3′ 5′ TGG TAG AGA AGG ACG GAG AG 3′ 188 GA 60° C. WMS154 5′ TCA CAGAGA GAG AGG GAG GG 3′ 5′ ATG TGT ACA TGT TGC CTG CA 3′ 102 GA 55° C.WMS155 5′ CAA TCA TTT CCC CCT CCC 3′ 5′ AAT CAT TGG AAA TCC ATA TGC 141CT 60° C. C 3′ WMS156 5′ CCA ACC GTG CTA TTA GTC ATT C 3′ 5′ CAA TGC AGGCCC TCC TAA C 3′ 277 GT 60° C. WMS157 5′ GTC GTC GCG GTA AGC TTG 3′ 5′GAG TGA ACA CAC GAG GCT TG 3′ 106 CT 60° C. WMS159 5′ GGG CCA ACA CTGGAA CAC 3′ 5′ GCA GAA GCT TGT TGG TAG GC 3′ 192 GT 60° C. WMS160 5′ TTCAAT TCA GTC TTG GCT TGG 3′ 5′ CTG CAG GAA AAA AAG TAC ACC 184 GA 60° C.C 3′ WMS161 5′ GAT CGA GTG ATG GCA GAT GG 3′ 5′ TGT GAA TTA CTT GGA CGTGG 3′ 154 CT 60° C. WMS162 5′ AGT GGA TCG ACA AGG CTC TG 3′ 5′ AGA AGAAGC AAA GCC TTC CC 3′ 208 CA 60° C. WMS163 5′ ACC TCG ACA GAC CTG GTA CG3′ 5′ GTC TTT GTC ACC CGA TGG AC 3′ 127 CT 55° C. WMS164 5′ ACA TTT CTCCCC CAT CGT C 3′ 5′ TTG TAA ACA AAT CGC ATG CG 3′ 120 CT 55° C. WMS1655′ TGC AGT GGT CAG ATG TTT CC 3′ 5′ CTT TTC TTT CAG ATT GCG CC 3′ 199 GA60° C. WMS169 5′ ACC ACT GCA GAG AAC ACA TAC G 3′ 5′ GTG CTC TGC TCT AAGTGT GGG 3′ 196 GA 60° C. WMS174 5′ GGG TTC CTA TCT GGT AAA TCC C 3′ 5′GAC ACA CAT GTT CCT GCC AC 3′ 173 CT 55° C. WMS179 5′ AAG TTG AGT TGATGC GGG AG 3′ 5′ CCA TGA CCA GCA TCC ACT C 3′ 181 GT 55° C. WMS180 5′ATC CGC CTA AGG AAT AGT GT 3′ 5′ GAT CGC ACG GGA GAG AGA G 3′ 84 CT 50°CWMS181 5′ TCA TTG GTA ATG AGG AGA GA 3′ 5′ GAA CCA TTC ATG TGC ATG TC 3′135 GA 50°C WMS182 5′ TGA TGT AGT GAG CCC ATA GGC 3′ 5′ TTG CAC ACA GCCAAA TAA GG 3′ 165 CT 60° C. WMS186 5′ GCA GAG CCT GGT TCA AAA AG 3′ 5′CGC CTC TAG CGA GAG CTA TG 5′ 140 GA 60° C. WMS189 5′ AGG AGC AGC GGAACG AAC 3′ 5′ AGA AAT ACG GAA ACC CAC AA 3′ 117 CA 55° C. WMS190 5′ GTGCTT GCT GAG CTA TGA GTC 3′ 5′ GTG CCA CGT ACC TTT G 3′ >201 CT,GT 60° C.WMS191 5′ AGA CTG TTG TTT GCG GGC 3′ 5′ TAG CAC GAC AGT TGT ATG CAT 128CT 60° C. G 3′ WMS192 5′ GGT TTT CTT TCA GAT TGC GC 3′ 5′ CGT TGT CTAATC TTG CCT TGC 3′ 191 CT 60° C. WMS193 5′ CTT TGT GCA CCT CTC TCT CC 3′5′ AAT TGT GTT GAT GAT TTG GGG 3′ 171 CT,CA 60° C. WMS194 5′ GAT CTG CTCTAC TCT CCT CC 3′ 5′ CGA CGC AGA ACT TAA ACA AG 3′ 131 CT 50°C WMS195 5′AGG TGC CGT CGC GTC TAC 3′ 5′ ACC CCC CAC GTC AGA GAG 3′ 108 CT 60° C.WMS197 5′ GAG AAA GAG GTC TGG AGG TCG 3′ 5′ CAA AAT GCA CAA GAA TGG AGG3′ 126 CT 60° C. WMS198 5′ TTG AAC CGG AAG GAG TAC AG 3′ 5′ TCA GTT TATTTT GGG CAT GTG 3′ 130 CA 60° C. WMS200 5′ TCA ACG GAA CAG ATG AGC G 3′5′ GAC CTG ATG AGA GCA AGC AC 3′ 250 CT 60° C. WMS203 5′ CCC AAA GCA GCGCAA GC 3′ 5′ ACC AAT GCT ATC GGC TCG 3′ 139 CA,GA 55° C. WMS205 5′ CGACCC GGT TCA CTT CAG 3′ 5′ AGT CGC CGT TGT ATA GTG CC 3′ 152 CT 60° C.WMS210 5′ TGC ATC AAG AAT AGT GTG GAA G 3′ 5′ TGA GAG GAA GGC TCA CAC CT3′ 192 GA 60° C. WMS212 5′ AAG CAA CAT TTG CTG CAA TG 3′ 5′ TGC AGT TAACTT GTT GAA AGG 104 CT 60° C. A 3′ WMS213 5′ TGC CTG GCT CGT TCT ATC TC3′ 5′ CTA GCT TAG CAC TGT CGC CC 3′ 184 GA 60° C. WMS218 5′ CGG CAA ACGGAT ATC GAC 3′ 5′ AAC AGT AAC TCT CGC CAT AGC 149 CT 60° C. C 3′ WMS2195′ GAT GAG CGA CAC CTA GCC TC 3′ 5′ GGG GTC CGA GTC CAC AAC 3′ 181 GAimp60° C. WMS224 5′ TGA GTC CAG CAC TGC TGC 3′ 5′ CAA CAT CCG CTC GTA TTCAA 3′ 142 CT 50°C WMS228 5′ TCA TAT GCA CCT CTT TCC TAG G 3′ 5′ GTG TGCCAC CTT TGA CGT C 3′ 210 CT,CA 60° C. WMS231 5′ AGC TCG GGA TGA AGC GTG3′ 5′ GAT CCG CCG CTG CGT TT 3′ 130 GAimp 60° C. WMS232 5′ ATC TCA ACGGCA AGC CG 3′ 5′ CTG ATG CAA GCA ATC CAC C 3′ 141 GA 55° C. WMS233 5′TCA AAA CAT AAA TGT TCA TTG GA 3′ 5′ TCA ACC GTG TGT ATT TTT GTC C 3′261 CT 60° C. WMS234 5′ GAG TCC TGA TGT GAA GCT GTT G 3′ 5′ CTC ATT GGGGTG TGT ACG TG 3′ 241 CT,CA 55° C. WMS237 5′ GAA TCA CTT GTG AAG CAT CTGG 3′ 5′ CTG GAT GCA TCA CAT CCA AC 3′ 137 CT 55° C. WMS238 5′ TCG CTTCTA CCG CTC ACC 3′ 5′ AGT GCC TTG CCG AGG TC 3′ 204 CT,GT,GGGT 55° C.WMS241 5′ TCT TCC AAC TAA AGC ATA GC 3′ 5′ CTT CCA TGG ACT ACA TAC TAG146 GA 55° C. C 3′ WMS242 5′ TCC AGG GCA GTA GGC AGG 3′ 5′ TGT TGT TGGCCT GTA TGC AT 3′ 142 GA 55° C. WMS244 5′ GGC AGC TGA GGC AAT CTG 3′ 5′TTT GGA CAT TTC CCA GCG 3′ 227 GAimp 60° C. WMS245 5′ CAG CGC AGT TAGCTC GC 3′ 5′ ATC TGT CCA TTC GAG CGC 3′ 141 CT 60° C. WMS247 5′ GCA ATCTTT TTT CTG ACC ACG 3′ 5′ ATG TGC ATG TCG GAC GC 3′ 158 GA 60° C. WMS2485′ AGG ACT TCC GCA CCC TG 3′ 5′ TGG CGT GGT CTA AAT GGA C 3′ 185 CA 60°C. WMS249 5′ CAA ATG GAT CGA GAA AGG GA 3′ 5′ CTG CCA TTT TTC TGG ATCTAC 177 GAimp 60° C. C 3′ WMS251 5′ CAA CTG GTT GCT ACA CAA GCA 3′ 5′GGG ATG TCT GTT CCA TCT TAG 3′ 103 CA 55° C. WMS255 5′ CAA CTG TAC GTAGGT TTC ATT GC 3′ 5′ TCT GCC GTA AGT CGC CTC 3′ 148 GA 55° C. WMS257 5′AGA GTG CAT GGT GGG ACG 3′ 5′ CCA AGA CGA TGC TGA AGT CA 3′ 192 GT 60°C. WMS258 5′ GAT CGC TTC ATC TCT CTC TCT C 3′ 5′ GTA CAC GCC GTA GGC CC3′ >81 CT 60° C. WMS259 5′ AGG GAA AAG ACA TCT TTT TTT TC 3′ 5′ CGA CCGACT TCG GGT TC 3′ 105 GA 55° C. WMS260 5′ GCC CCC TTG CAC AAA TC 3′ 5′CGC AGC TAC AGG CC3′ 157 GA 55° C. WMS261 5′ CTC CCT GTA CGC CTA AGG C3′ 5′ CTC GCG CTA GCC ATT G 3′ 192 CT 55° C. WMS263 5′ TCT GCC GTA AGTCGC CTC 3′ 5′ GGT TTC ATT GCT TGC CCT AA 3′ 134 CT 55° C. WMS264 5′ GAGAAA CAT GCC GAA CAA CA 3′ 5′ GCA TGC ATG AGA ATA GGA ACT 219 CA 60° C. G3′ WMS265 5′ TGT TGC GGA TGG TCA CTA TT 3′ 5′ GAG TAC ACA TTT GGC CTCTGC 3′ 200 GT 55° C. WMS268 5′ AGG GGA TAT GTT GTC ACT CCA 3′ 5′ TTA TGTTGC GTA CGT ACC C 3′ 241 GAimp 55° C. WMS269 5′ TGC ATA TAA ACA GTC ACACAC CC 3′ 5′ TTT GAG CTC CAA AGT GAG TTA >148 CA 60° C. GC 3′ WMS271 5′CAA GAT CGT GGA GCC AGC 3′ 5′ AGC TGC TAG CTT TTG GGA CA 3′ 162 CT,GA60° C. WMS272 5′ TGC TCT TTG GCG AAT ATA TGG 3′ 5′ GTT CAA AAC AAA TTAGGC AAA 140 CA 55° C. CC 3′ WMS273 5′ ATT GGA CGG ACA GAT GCT TT 3′ 5′AGC AGT GAG GAA GGG GAT C 3′ 167 GA 55° C. WMS274 5′ AAC TTG CAA AAC TGTTCT GA 3′ 5′ TAT TTG AAG CGG TTT GAT TT 3′ 179 GT 50°C WMS275 5′ AAT TTTCTT CAC TTA TTC T 3′ 5′ AAC AAA AAA TTA GGG CC 3′ 107 CT 50°C WMS276 5′ATT TGC CTG AAG AAA ATA TT 3′ 5′ AAT TTC ACT GCA TAC ACA AG 3′ 99 CT 55°C. WMS278 5′ GTT GCT TCA TGA ACG CTC AA 3′ 5′ CTG CCC AAT TTT CTC CAC TC3′ 241 GTimpGAimp 55° C. WMS281 5′ CGG CCA TAT TTC TGT AAG TAT GC 3′ 5′GCA GGT AAT GGC CGG AC 3′ 135 GT 60° C. WMS282 5′ TTG GCC GTG TAA GGC AG3′ 5′ TCT CAT TCA CAC ACA ACA CTA 220 GA 55° C. GC 3′ WMS284 5′ AAT GAAAAA ACA CTT GCG TGG 3′ 5′ GCA CAT TTT TCA CTT TCG GG 3′ 123 GA 60° C.WMS285 5′ ATG ACC CTT CTG CCA AAC AC 3′ 5′ ATC GAC CGG GAT CTA GCC 3′243 GA 60° C. WMS291 5′ CAT CCC TAC GCC ACT CTG C 3′ 5′ AAT GGT ATC TATTCC GAC CCG 3′ >158 CA 60° C. WMS292 5′ TCA CCG TGG TCA CCG AC 3′ 5′ CCACCG AGC CGA TAA TGT AC 3′ 220 CT 60° C. WMS293 5′ TAC TGG TTC ACA TTGGTG CG 3′ 5′ TCG CCA TCA CTC GTT CAA G 3′ 201 CA 55° C. WMS294 5′ GGATTG GAG TTA AGA GAG AAC CG 3′ 5′ GCA GAG TGA TCA ATG CCA GA 3′ 100 GAimp55° C. WMS295 5′ GTG AAG CAG ACC CAC AAC AC 3′ 5′ GAC GGC TGC GAC GTAGAG 3′ 258 GA 60° C. WMS296 5′ AAT TCA ACC TAC CAA TCT CTG 3′ 5′ GCC TAATAA ACT GAA AAC GAG 3′ 149 CT 55° C. WMS297 5′ ATC GTC ACG TAT TTT GCAATG 3′ 5′ TGC GTA AGT CTA GCA TTT TCT 150 GT,GA 55° C. G 3′ WMS299 5′ACT ACT TAG GCC TCC CGC C 3′ 5′ TGA CCC ACT TGC AAT TCA TC 3′ 208 GA,TAG55° C. WMS301 5′ GAG GAG TAA GAC ACA TGC CC 3′ 5′ GTG GCT GGA GAT TCAGGT TC 3′ 204 GA,G 55° C. WMS302 5′ GCA AGA AGC AAC AGC AGT AAC 3′ 5′CAG ATG CTC TTC TCT GCT GG 3′ 180 GA 60° C. (340) WMS304 5′ AGG AAA CAGAAA TAT CGC GG 3′ 5′ AGG ACT GTG GGG AAT GAA TG 3′ 217 CT 55° C. WMS3115′ TCA CGT GGA AGA CGC TCC 3′ 5′ CTA CGT GCA CCA CCA TTT TG 3′ 151 GA60° C. WMS312 5′ ATC GCA TGA TGC ACG TAG AG 3′ 5′ ACA TGC ATG CCT ACCTAA TGG 3′ 235 GA 60° C. WMS313 5′ CCG CCC TCA TTA AGT TTC AC 3′ 5′ TTTGAC AAG TAC ACG AGT CTG 156 CT,GT 55° C. C 3′ WMS314 5′ AGG AGC TCC TCTGTG CCA C 3′ 5′ TTC GGG ACT CTC TTC CCT G 3′ 170 CT 55° C. WMS316 5′ CATGGA CAT TTT ACC ACA AGA C 3′ 5′ TGC GTG TGG TCC ACC TC 3′ 176 AT,GT 55°C. WMS319 5′ GGT TGC TGT ACA AGT GTT CAC G 3′ 5′ CGG GTG CTG TGT GTA ATGAC 3′ 200 CT 55° C. WMS320 5′ CGA GAT ACT ATG GAA GGT GAG G 3′ 5′ ATCTTT GCA AGG ATT GCC C 3′ >263 GT,GA 55° C. WMS321 5′ CAA TGT GGA GAC GGTGTG C 3′ 5′ TGT TGC ATG CGA TCA TGC 3′ 265 GT,GAimp 60° C. WMS322 5′ TCACAA AAT GAT TTC TCA TCC G 3′ 5′ TGC AGA AAA CCA ACA AGG G 3′ 119 GA 55°C. WMS325 5′ TTT CTT CTG TCG TTC TCT TCC C 3′ 5′ TTT TTA CGC GTC AAC GACG 3′ 131 CT 55° C. WMS328 5′ GCA ATC CAC GAG AAG AGA GG 3′ 5′ CAC AAACTC TTG ACA TGT GCG 3′ 193 GT 55° C. WMS330 5′ TTG CTA TCC ATG TGC CAGAG 3′ 5′ ACA TGT TTC ATG CAG GTA GCC 3′ 165 GTT 55° C. WMS332 5′ AGC CAGCAA GTC ACC AAA AC 3′ 5′ AGT GCT GGA AAG AGT AGT GAA 231 GA 60° C. GC 3′WMS333 5′ GCC CGG TCA TGT AAA ACG 3′ 5′ TTT CAG TTT GCG TTA AGC TTT 150GA 55° C. G 3′ WMS334 5′ AAT TTC AAA AAG GAG AGA GA 3′ 5′ AAC ATG TGTTTT TAG CTA TC 3′ 123 GA 50°C WMS335 5′ CGT ACT CCA CTC CAC ACG G 3′ 5′CGG TCC AAG TGC TAC CTT TC 3′ 187 GA,GCGT 55° C. (225) WMS336 5′ CCC TTTAAT CTC GCT CCC TC 3′ 5′ GTC TCT TTC TCG TAC TTC CAG 108 CT 55° C. G 3′WMS337 5′ CCT CIT CCT CCC TCA CTT AGC 3′ 5′ TGC TAA CTG GCC TTT GCC 3′183 CT,CACT,CA 55° C. WMS339 5′ AAT TTT CTT CCT CAC TTA TT 3′ 5′ AAA CGAACA ACC ACT CAA TC 3′ 159 CT 50°C WMS340 5′ GCA ATC TTT TTT CTG ACC ACG3′ 5′ ACG AGG CAA GAA CAC ACA TG 3′ 132 GA 60° C. WMS341 5′ TTC AGT GGTAGC GGT CGA G 3′ 5′ CCG ACA TCT CAT GGA TCC AC 3′ 133 CT 55° C. (150)WMS342 5′ TAT CCA GAG CAG ACG GAC G 3′ 5′ GGT CTA GCT TCG ACG ACA CC 3′169 GT 55° C. WMS344 5′ CAA GGA AAT AGG CGG TAA CT 3′ 5′ ATT TGA GTC TGAAGT TTG CA 3′ 131 GT 55° C. WMS346 5′ CAA GCA AGG TTT CGT TTT ATC C 3′5′ GCA TGT GGT CCA TGT ACT GC 3′ 203 AT,GT 55° C. WMS349 5′ GGC TTC CAGAAA ACA ACA GG 3′ 5′ ATC GGT GCG TAC CAT CCT AC 3′ 230 GA 55° C. WMS3505′ ACC TCA TCC ACA TGT TCT ACG 3′ 5′ GCA TGG ATA GGA CGC CC 3′ 146 GT55° C. WMS353 5′ CCA TGT TGA GTA GGT TCA GCC 3′ 5′ CTT GGC CAG AAG CTACGA AC 3′ 179 GCGT,GT 60° C. WMS356 5′ AGC GTT CTT GGG AAT TAG AGA 3′ 5′CCA ATC AGC CTG CAA CAA C 3′ 224 GA 55° C. WMS357 5′ TAT GGT CAA AGT TGGACC TCG 3′ 5′ ACG CTG CAG CTC TTC TTC AG 3′ 123 GA 55° C. WMS358 5′ AAACAG CGG ATT TCA TCG AG 3′ 5′ TCC GCT GTT CTG ATC TC 3′ 164 GAimp 55° C.WMS359 5′ CTA ATT GCA ACA GGT CAT GGG 3′ 5′ TAC TTG TGT TCT GGG ACA ATG217 CT,CTTimp 55° C. G 3′ WMS361 5′ GTA ACT TGT TGC CAA AGG GG 3′ 5′ ACAAAG TGG CAA AAG GAG ACA 3′ 126 GAimp 60° C. WMS368 5′ CCA TTT CAC CTAATG CCT GC 3′ 5′ AAT AAA ACC ATG AGC TCA CTT 249 AT 60° C. GC 3′ WMS3695′ CTG CAG GCC ATG ATG ATG 3′ 5′ ACC GTG GGT GTT GTG AGC 3′ 188 CTimp60° C. WMS371 5′ GAC CAA GAT ATT CAA ACT GGC C 3′ 5′ AGC TCA GCT TGC TTGGTA CC 3′ 170 CA,GA 60° C. WMS372 5′ AAT AGA GCC CTG GGA CTG GG 3′ 5′GAA GGA CGA CAT TCC ACC TG 3′ >329 GA 60° C. WMS374 5′ ATA GTG TGT TGCATG CTG TGT G 3′ 5′ TCT AAT TAG CGT TGG CTG CC 3′ 213 GT 60° C. WMS3755′ ATT GGC GAC TCT AGC ATA TAC G 3′ 5′ GGG ATG TCT GTT CCA TCT TAG 156CA 55° C. C 3′ WMS376 5′ GGG CTA GAA AAC AGG AAG GC 3′ 5′ TCT CCC GGAGGG TAG GAG 3′ 147 CA, GAimp 60° C. WMS382 5′ GTC AGA TAA CGC CGT CCA AT3′ 5′ CTA CGT GCA CCA CCA TTT TG 3′ 115 GA 60° C. WMS383 5′ ACG CCA GTTGAT CCG TAA AC 3′ 5′ GAC ATC AAT AAC CGT GGA TGG 3′ 195 GT 60° C. WMS3845′ TTT TCA TTG TGC CCT CTA CT 3′ 5′ GCC AAG TTT CTT AGC TAG TTA 204GTimp 55° C. A 3′ WMS388 5′ CTA CAA TTC GAA GGA GAG GGG 3′ 5′ CAC CGCGTC AAC TAC TTA AGC 3′ 162 CT,CA,CA 60° C. WMS389 5′ ATC ATG TCG ATC TCCTTG ACG 3′ 5′ TGC CAT GCA CAT TAG CAG AT 3′ 130 CT,GT 60° C. WMS390 5′AAG TTT CAC ACA AGA TCT CTC C 3′ 5′ TGA CAA GTA CAC GAG TCT GC 3′ 143CT,GT 55° C. WMS391 5′ ATA GCG AAG TCT CCC TAC TCC A 3′ 5′ ATG TCG ATGTCG GAC GC 3′ 150 CA,GA 55° C. WMS393 5′ TCA TCT GCT ATT TGT GCT ACA 3′5′ TCA AAT ACA CCA ATG TGC C 3′ 107 CA 55° C. WMS395 5′ TAC AAC CGC AAGTAA TGC CA 3′ 5′ TAC CAA CAC CCT AGC CCT TG 3′ 147 CA 60° C. WMS397 5′TGT CAT GGA TTA TTT GGT CGG 3′ 5′ CTG CAC TCT CGG TAT ACC AGC 3′ 179 CT55° C. WMS400 5′ GTG CTG CCA CCA CTT GC 3′ 5′ TGT AGG CAC TGC TTG GGA G3′ 139 CA 60° C. WMS403 5′ CGA CAT TGG CTT CGG TG 3′ 5′ ATA AAA CAG TGCGGT CCA GG 3′ 133 CA 55° C. WMS408 5′ TCG ATT TAT TTG GGC CAC TG 3′ 5′GTA TAA TTC GTT CAC AGC ACG 176 CA 55° C. C 3′ WMS410 5′ GCT TGA GAC CGGCAC AGT 3′ 5′ CGA GAC CTT GAG GGT CTA GA 3′ 334 CA 55° C. WMS411 5′ CCCATA CGA TGA TGT GGT TCC 3′ 5′ CAA ACG GAA CAT GGT CCC 3′ 148 CT 55° C.WMS412 5′ ATC AAC AAG GTT TGT GTG TTG G 3′ 5′ ATG AAA CGC GAC CTC CC 3′121 GA 55° C. WMS413 5′ TGC TTG TCT AGA TTG CTT GGG 3′ 5′ GAT CGT CTCGTC CTT GGC A 3′ 94 GA 60° C. WMS415 5′ GAT CTC CCA TGT CCG CC 3′ 5′ CGACAG TCG TCA CTT GCC TA 3′ 131 GAimp 55° C. WMS425 5′ GAG CCC ACA AGC TGGCA 3′ 5′ TCG TTC TCC CAA GGC TTG 3′ >143 CT 60° C. WMS427 5′ AAA CTT AGAACT GTA ATT TCA GA 3′ 5′ AGT GTG TTC ATT TGA CAG TT 3′ 215 CA 50°CWMS428 5′ CGA GGC AGC GAG GAT TT 3′ 5′ TTC TCC ACT AGC CCC GC 3′ 143 GA60° C. WMS429 5′ TTG TAC ATT AAG TTC CCA TTA 3′ 5′ TTT AAG GAC CTA CATGAC AC 3′ 221 CT 50°C (290) WMS434 5′ ATG AGT TCC GCC AAA GAA TG 3′ 5′ACG AAA TAC ACA AGT GGG ACA 3′ 216 GT 55° C. WMS437 5′ GAT CAA GAC TTTTGT ATC TCT C 3′ 5′ GAT GTC CAA CAG TTA GCT TA 3′ 109 CT 50°C WMS440 5′CCT ATG GTC TCC ATC ATG AGG 3′ 5′ TCA TGT CAA CTC AAG AAC ACG 3′ 112 CT55° C. WMS443 5′ GGG TCT TCA TCC GGA ACT CT 3′ 5′ CCA TGA TTT ATA AATTCC ACC 3′ 134 CA,GA 55° C. WMS445 5′ TTT GTT GGG GGT TAG GAT TAG 3′ 5′CCT TAA CAC TTG CTG GTA GTG 192 CT 55° C. A 3′ WMS448 5′ AAA CCA TAT TGGGAG GAA AGG 3′ 5′ CAC ATG GCA TCA CAT TTG TG 3′ 231 GA 60° C. WMS455 5′ATTT CGG TTC GCT AGC TAC CA 3′ 5′ ACG GAG AGC AAC CTG CC 3′ 151 GTimp55° C. WMS456 5′ TCT GAA CAT TAC ACA ACC CTG A 3′ 5′ TGC TCT CTC TGA ACCTGA AGC 3′ 132 GA 55° C. WMS458 5′ AAT GGC AAT TGG AAG ACA TAG C 3′ 5′TTC GCA ATG TTG ATT TGG C 3′ 113 CA 60° C. WMS459 5′ ATG GAG TGG TCA CACTTT GAA 3′ 5′ AGC TTC TCT GAC CAA CTT CTC >138 GA 55° C. G 3′ WMS469 5′CAA CTC AGT GCT CAC ACA ACG 3′ 5′ CGA TAA CCA CTC ATC CAC ACC 3′ >156 CT60° C. WMS471 5′ CGG CCC TAT CAT GGC TG 3′ 5′ GCT TGC AAG TTC CAT TTT GC3′ 149 CA 60° C. WMS473 5′ TCA TAC GGG TAT GGT TGG AC 3′ 5′ CAC CCC CTTGTT GGT CAC 3′ 220 GTimp 55° C. WMS476 5′ ATG GGT TCG TAC TAA CAT CAG C3′ 5′ TTG CTG GTA GCT TCA ATC CC 3′ >194 GTimp 60° C. WMS480 5′ TGC TGCTAC TTG TAC AGA GGA C 3′ 5′ CCG AAT TGT CCG CCA TAG 3′ 188 CT,CA 60° C.WMS484 5′ ACA TCG CTC TTC ACA AAC CC3′ 5′ AGT TCC GGT CAT GGC TAG G 3′145 CT 55° C. WMS494 5′ ATT GAA CAG GAA GAC ATC AGG G 3′ 5′ TTC CTG GAGCTG TCT GGC 3′ 198 CA 60° C. WMS495 5′ GAG AGC CTC GCG AAA TAT AGG 3′ 5′TGC TTC TGG TGT TCC TTC G 3′ 168 GA 60° C. WMS497 5′ GTA GTG AAG ACA AGGGCA TT 3′ 5′ CCG AAA GTT GGG TGA TAT AC 3′ >106 GTimp 55° C. WMS499 5′ACT TGT ATG CTC CAT TGA TTG G 3′ 5′ GGG GAG TGG AAA CTG CAT AA 3′ 145 GA60° C. WMS501 5′ GGC TAT CTC TGG CGC TAA AA 3′ 5′ TCC ACA AAC AAG TAGCGC C 3′ 172 CA 60° C. WMS512 5′ AGC CAC CAT CAG CAA AAA TT 3′ 5′ GAACAT GAG CAG TTT GGC AC 3′ 185 GT 60° C. WM5513 5′ ATC CGT AGC ACC TACTGG TCA 3′ 5′ GGT CTG TTC ATG CCA CAT TG 3′ 144 CA 60° C. WMS515 5′ AACACA ATG GCA AAT GCA GA 3′ 5′ CCT TCC TAG TAA GTG TGC CTC 134 GTimp 60°C. A 3′ WMS518 5′ AAT CAC AAC AAG GCG TGA CA 3′ 5′ CAG GGT GGT GCA TGCAT 3′ 166 CA 55° C. WMS530 5′ AAA TAG GAC AAC CCA CGG C 3′ 5′ TCA ACTTCT TGG CCT CCA TC 3′ 186 CT 55° C. WMS532 5′ ACT GCG TGT GCC TAC AAT TG3′ 5′ TCA CTC GCA CTC GAT AGG C 3′ 142 GT 60° C. WMS533 5′ AAG GCG AATCAA ACG GAA TA 3′ 5′ GTT GCT TTA GGG GAA AAG CC 3′ 147 CT,CA 60° C.WMS537 5′ ACA TAA TGC TTC CTG TGC ACC 3′ 5′ GCC ACT TTT GTG TCG TTC CT3′ 209 CA,TA 60° C. WMS538 5′ GCA TTT CGG GTG AAC CC 3′ 5′ GTT GCA TGTATA CGT TAA GCG 147 GTimp 60° C. G 3′ WMS540 5′ TCT CGC TGT GAA ATC CTATTT C 3′ 5′ AGG CAT GGA TAG AGG GGC 3′ 129 CTimp 55° C. WMS544 5′ TAGAAT TCT TTA TGG GGT CTG C 3′ 5′ AGG ATT CCA ATC CTT CAA AAT 167CT,ATCT,CT 55° C. T 3′ WMS550 5′ CCC ACA AGA ACC TTT GAA GA 3′ 5′ CATTGT GTG TGC AAG GCA C 3′ 150 CT, GT 55° C. WMS554 5′ TGC CCA CAA CGG AACTTG 3′ 5′ GCA ACC ACC AAG CAC AAA GT 3′ 160 CT,GTimp 60° C. WMS565 5′GCG TCA GAT ATG CCT ACC TAG G 3′ 5′ AGT GAG TTA GCC CTG AGC CA 3′ 142 CA60° C. WMS566 5′ TCT GTC TAC CCA TGG GAT TTG 3′ 5′ CTG GCT TCG AGG TAAGCA AC 3′ 130 CA,TA 60° C. WMS569 5′ GGA AAC TTA TTG ATT GAA AT 3′ 5′TCA ATT TTG ACA GAA GAA TT 3′ 134 GT 47°C WMS570 5′ TCG CCT TTT ACA GTCGGC 3′ 5′ ATG GGT AGC TGA GAG CCA AA 3′ 143 CT,GT 60° C. WMS573 5′ AAGAGA TAA CAT GCA AGA AA 3′ 5′ TTC AAA TAT GTG GGA ACT AC 3′ 212 CA 50°CWMS577 5′ ATG GCA TAA TTT GGT GAA ATT G 3′ 5′ TGT TTC AAG CCC AAC TTCTAT 133 CA,TA 55° C. T 3′ WMS582 5′ AAG CAC TAC GAA AAT ATG AC 3′ 5′ TCTTAA GGG GTG TTA TCA TA 3′ 151 CA 50°C WMS583 5′ TTC ACA CCC AAC CAA TAGCA 3′ 5′ TCT AGG CAG ACA CAT GCC TG 3′ 165 CA 60° C. WMS588 5′ GAT CCCCAA TTG CAT GTT G 3′ 5′ CTT GCA ACT GGG GGA CAC 3′ 102 GT 60° C.

[0008] These markers are distinguished by a high degree of polymorphismbetween different wheat varieties or lines and usually detect severalalleles per genetic locus in different wheat lines.

[0009] They can therefore be used for DNA fingerprinting, speciesidentification, relationship or similarity studies, characterization ofcytological lines, such as deletion lines, substitution lines, additionlines, etc. and all forms of genetic mappings, including mapping ofindividual genes and quantitative distinguishing features (QTLs). Inaddition, their use is also very suitable for automation and it ispossible to carry out the detection of the products with nonradioactivemethods.

[0010] With the help of this inventive marker, the possibility isprovided, for example, of differentiating almost all European wheatlines.

[0011] The invention is described in greater detail below by means ofexamples.

1. Amplification of the Microsatellite Markers

[0012] The microsatellite markers are amplified according to thefollowing protocol:

[0013] 10 mM tris-HCI, pH 8 50 mM KCI 1.5 mM MgCl₂ (in a few exceptionalcases 3 mM MgCl₂) 0.01% (w/v) gelatin 0.2 mM of each desoxynucleotide250 nM of each primer (in each case to the left and right of a pair) 1-2units taq polymerase 50-150 ng matrixes (template) DNA

[0014] are amplified in a volume of 25 or 50 IL according to thefollowing profile: 92° C. 3 minute 92° C. 1 minute (denaturing phase)60° C. 1 minutes (annealing phase) 45 cycles 72° C. 2 minutes(elongation phase) 72° C. 10 minutes (extension phase)

[0015] The amplification takes place in a Perkin Elmer 9600 with lidheating or in an MJ Research Thermocycler without lid heating. In thisapparatus, a layer of mineral oil is placed over the reactions. Thetemperature of the annealing phase depends on the melting point (Tm) ofthe primer and in some cases even is 50° C or 55° C.

2. Separation of the Microsatellite Markers on Polyacrylamide Gels,Which Are Not Denaturing

[0016] The PCR reactions are mixed with {fraction (1/10)} volume of stopbuffer (0.02 M tris acetate of pH 8.1, 0.025 M sodium acetate, 0.02 MEDTA, 70% glycerin, 0.2% SDS, 0.6% bromphenol blue, 0.6% xylene cyanol)and in each case 25 μL are separated in 10% polyacrylamide gels (1.5 mmthick, 18 cm long).

[0017] Formulation for polyacrylamide gel (10%): 25 mL stock acrylamidesolution (19 g acrylamide, 1 g bisacrylamide, diluted to 100 mL withwater) 10 mL 5X TBE (1X TBE = 0.09 M tris borate of pH 8.3, 0.002 M EDTA15 mL water

[0018] are mixed and the polymerization is started by the addition of220 μL of ammonium persulfate (10%, freshly prepared) and 20 μL ofTEMED. Immediately after the addition, the mixture is poured into thesealed gel mold and the comb for forming pockets is inserted. Thepolymerization is completed after about 1 hour. The gel is placed in thegel chamber and a preliminary run is carried out without samples forabout 30 minutes at 150 volts in IX TBE. After that, the samples areloaded (25 μL of each) and the separation is carried out for 14-16 hoursat 100 volts.

[0019] After the electrophoresis is completed, the gel is stained forabout minutes in ethidium bromide (1-2 drops of 10 mg/mL in 1 liter ofwater) and the fragments are made visible by a UV transilluminator anddocumented.

3. Separation of Microsatellite Markers on Denaturing Gels

[0020] For the separation of the amplified fragments on denaturing gels,an automatic laser fluorescence (A.L.F.) sequencer (Pharmacia), forexample, is used. In order to enable the fragments to be detected bymeans of a laser, one primer per pair is marked at the 5′ end withfluorescein. Per PCR reaction, 0.3 to 1.5 microliters are mixed with 2.5microliters of stop buffer (deionized formamide; 5 mg/mL dextran blue),denatured (1 minute; 90° C.) and loaded onto the gel. Gel plates with a9 cm separation distance are used, as recommended by the manufacturerfor the fragment analysis. The gel solution contains 6.5% Long-Ranger(AT Biochem), 7M urea and 1.2X TBE buffer. The gels are 0.35 or 0.5 mmthick. The conditions for the gel run are 600 V, 40 mA, 50 W, 0.84 sdata collection interval and 2 mW laser energy. The gel runs are endedafter about 80 to 90 minuutes. This is sufficient for detectingfragments up to a size of 300 bp. A gel can be used for four or fiveruns. For each gel run, a data set is obtained. With this data and bymeans of internal size standards, the exact fragment sizes sredetermined in the computer program Fragment Manager (Pharmacia) and thusthe smallest size differences of a base pair are deterrnined.

1. Microsatellite markers (based on hypervariable genome sections) forplants of the Triticum aestivum species, as well as of the TribeTriticeae using the polymerase chain reaction (PCR), characterized inthat a sequence tagged site (STS), which is defined by two specificprimers, which average a length of 20±3 bases and flank a microsatellitesequence, which microsatellite markers are amplified to polymorphisms(PCR products of different length).
 2. The microsatellite markers ofclaim 1, characterized in that the microsatellite sequence is atandem-repetitive n-fold repetition of a di-, tri- or tetranucleotidesequence, in which n>10.
 3. The microsatellite markers of claim 1,characterized in that the microsatellite sequence is a compositemicrosatellite sequence.
 4. The microsatellite markers of claim 1,characterized in that the microsatellite sequence is an imperfectsequence, in which individual bases are mutated.
 5. The microsatellitemarkers of claim 1, characterized in that the following primer pairswith assigned microsatellite sequences or a number thereof arecontained. WMS Number WMS Primer left WMS Primer Right Repeat TypeWMS-Nummer WMS Primer links WMS Primer rechts Repeat-Typ WMS052 5′ CTATGA GGC GGA GGT TGA AG 3′ 5′ TGC GGT CCT CTT CCA TTT 3′ GTimp WMS055 5′GCA TCT GGT ACA CTA GCT GCC 3′ 5′ TCA TGG ATG CAT CAC ATC CT 3 CTimpWMS057 5′ TCG ATT CTG AAA GGT TCA TCG 3′ 5′ CGA TCA AGT AGT TGA AAG CGC3′ AAAAAimp WMS058 5′ TCT GAT CCC GTG AGT GTA ACA 3′ 5′ GAA AAA AAT TGCATA TGA GCC C 3′ CA WMS060 5′ TGT CCT ACA CGG ACC ACG T 3′ 5′ GCA TTGACA GAT GCA CAC G 3′ CA WMS063 5′ TCG ACC TGA TCG CCC CTA 3′ 5′ CGC CCTGGG TGA TGA ATA GT 3′ GAA,CA,TA WMS067 5′ ACC ACA CAA ACA AGG TAA GCG 3′5′ CAA CCC TCT TAA TTT TGT TGG G 3′ CA WMS068 5′ AGG CCA GAA TCT GGG AATG 3′ 5′ CTC CCT AGA TGG GAG AAG GG 3′ GA WMS070 5′ AGT GGC TGG GAG AGTGTC AT 3′ 5′ GCC CAT TAC CGA GGA CAC 3′ GT WMS071 5′ GGC AGA GCA GCG AGACTC 3′ 5′ CAA GTG GAG CAT TAG GTA CAC G 3′ GT WMS077 5′ ACA AAG GTA AGCAGC ACC TG 3′ 5′ ACC CTC TTG CCC GTG TTG 3′ CA,GA WMS082 5′ ACG TTA GAAGGT GCA ATG GG 3′ 5′ AGT GGA TGC ACC GAC TTT G 3′ GT,GAimp WMS088 5′ CACTAC AAC TAT GCG CTC GC 3′ 5′ TCC ATT GGC TTC TCT CTC AA 3′ GT WMS095 5′GAT CAA ACA CAC ACC CCT CC 3′ 5′ AAT GCA AAG TGA AAA ACC CG 3′ CA WMS0995′ AAG ATG GAC GTA TGC ATC ACA 3′ 5′ GCC ATA TTT GAT GAC GCA TA 3′ CAWMS102 5′ TCT CCC ATC CAA CGC CTC 3′ 5′ TGT TGG TGG CTT GAC TAT TG 3′ CTWMS106 5′ CTG TTC TTG CGT GGC ATT AA 3′ 5′ AAT AAG GAC ACA ATT GGG ATG G3′ GA WMS107 5′ ATT AAT ACC TGA GGG AGG TGC 3′ 5′ GGT CTC AGG AGC AAGAAC AC 3′ CT WMS108 5′ CGA CAA TGG GGT CTT AGC AT 3′ 5′ TGC ACA CTT AAATTA CAT CCG C 3′ GTimp WMS111 5′ TCT GTA GGC TCT CTC CGA CTG 3′ 5′ ACCTGA TCA GAT CCC ACT CG 3′ CT,GT WMS112 5′ CTA AAC ACG ACA GCG GTG G 3′5′ GAT ATG TGA GCA GCG GTC AG 3′ CTimp WMS113 5′ ATT CGA GGT TAG GAG GAAGAG G 3′ 5′ GAG GGT CGG CCT ATA AGA CC 3′ GT WMS114 5′ ACA AAC AGA AAATCA AAA CCC G 3′ 5′ ATC CAT CGC CAT TGG AGT G 3′ GA WMS118 5′ GAT GTTGCC ACT TGA GCA TG 3′ 5′ GAT TAG TCA AAT GGA ACA CCC C 3′ CA WMS119 5′TGA CTA ACA TCC TTT GTC ACG C 3′ 5′ CAT GTC TCA ACC ACC CAC AG 3′ GTimpWMS120 5′ GAT CCA CCT TCC TCT CTC TC 3′ 5′ GAT TAT ACT GGT GCC GAA AC 3′CT,CA WMS121 5′ TCC TCT ACA AAC AAA CAC AC 3′ 5′ CTC GCA ACT AGA GGT GTATG 3′ CA WMS122 5′ GGG TGG GAG AAA GGA GAT G 3′ 5′ AAA CCA TCC TCC ATCCTG G 3′ CT,CA WMS124 5′ GCC ATG GCT ATC ACC CAG 3′ 5′ ACT GTT CGG TGCAAT TTG AG 3′ CT,GTimp WMS126 5′ CAC ACG CTC CAC CAT GAC 3′ 5′ GTT GAGTGG ATG CGG GAG G 3′ CA WMS128 5′ AGC ACA TTT TAA CAC AGA TA 3′ 5′ ATCTGT GAA ATT TTG AAA AC 3′ CA WMS129 5′ TCA GTG GGC AAG CTA CAC AG 3′ 5′AAA ACT TAG TAG CCG CGT 3′ GTimp WMS130 5′ AGC TCT GCT TCA CGA GGA AG 3′5′ CTC CTC TTT ATA TCG CGT CCC 3′ GT WMS131 5′ AAT CCC CAC CGA TTC TTCTC 3′ 5′ AGT TCG TGG GTC TCT GAT GG 3′ CT WMS132 5′ TAC CAA ATC GAA ACACAT CAG G 3′ 5′ CAT ATC AAG GTC TCC TTC CCC 3′ GA,GAA WMS133 5′ ATC TAAACA AGA CGG CGG TG 3′ 5′ ATC TGT GAC AAC CGG TGA GA 3′ CT WMS134 5′ CATGGA ACT TAG ACA GAA TGG 3′ 5′ CAG TAC TTG GTA CTG AAC AGG 3′ CA WMS1355′ TGT CAA CAT CGT TTT GAA AAG G 3′ 5′ ACA CTG TCA ACC TGG CAA TG 3′ GAWMS136 5′ GAC AGC ACC TTG CCC TTT G 3′ 5′ CAT CGG CAA CAT GCT CAT C 3′CT WMS140 5′ ATG GAG ATA TTT GGC CTA CAA C 3′ 5′ CTT GAC TTC AAG GCG TGACA 3′ CT WMS144 5′ TTT GCT GTG GTA CGA AAC ATA C 3′ 5′ ACT CAC AAA TGTCTA ATA AAA C 3′ GT WMS146 5′ CCA AAA AAA CTG CCT GCA TG 3′ 5′ CTC TGGCAT TGC TCC TTG G 3′ GAimp WMS148 5′ GTG AGG CAG CAA GAG AGA AA 3′ 5′CAA AGC TTG ACT CAG ACC AAA 3′ CA WMS149 5′ CAT TGT TTT CTG CCT CTA GCC3′ 5′ CTA GCA TCG AAC CTG AAC AAG 3′ GA WMS153 5′ GAT CTC GTC ACC CGGAAT TC 3′ 5′ TGG TAG AGA AGG ACG GAG AG 3′ GA WMS154 5′ TCA CAG AGA GAGAGG GAG GG 3′ 5′ ATG TGT ACA TGT TGC CTG CA 3′ GA WMS155 5′ CAA TCA TTTCCC CCT CCC 3′ 5′ AAT CAT TGG AAA TCC ATA TGC C 3′ CT WMS156 5′ CCA ACCGTG CTA TTA GTC ATT C 3′ 5′ CAA TGC AGG CCC TCC TAA C 3′ GT WMS157 5′GTC GTC GCG GTA AGC TTG 3′ 5′ GAG TGA ACA CAC GAG GCT TG 3′ CT WMS159 5′GGG CCA ACA CTG GAA CAC 3′ 5′ GCA GAA GCT TGT TGG TAG GC 3′ GT WMS160 5′TTC AAT TCA GTC TGG GCT TGG 3′ 5′ CTG CAG GAA AAA AAG TAC ACC C 3′ GAWMS161 5′ GAT CGA GTG ATG GCA GAT GG 3′ 5′ TGT GAA TTA CTT GGA CGT GG 3′CT WMS162 5′ AGT GGA TCG ACA AGG CTC TG 3′ 5′ AGA AGA AGC AAA GCC TTC CC3′ CA WMS163 5′ ACC TCG ACA GAC CTG GTA CG 3′ 5′ GTC TTT GTC ACC CGA TGGAC 3′ CT WMS164 5′ ACA TTT CTC CCC CAT CGT C 3′ 5′ TTG TAA ACA AAT CGCATG CG 3′ CT WMS165 5′ TGC AGT GGT CAG ATG TTT CC 3′ 5′ CTT TTC TTT CAGATT GCG CC 3′ GA WMS169 5′ ACC ACT GCA GAG AAC ACA TAC G 3′ 5′ GTG CTCTGC TCT AAG TGT GGG 3′ GA WMS174 5′ GGG TTC CTA TCT GGT AAA TCC C 3′ 5′GAC ACA CAT GTT CCT GCC AC 3′ CT WMS179 5′ AAG TTG AGT TGA TGC GGG AG 3′5′ CCA TGA CCA GCA TCC AGC C 3′ GT WMS180 5′ ATC CGC CTA AGG AAT AGT GT3′ 5′ GAT CGC ACG GGA GAG AGA 3′ CT WMS181 5′ TCA TTG GTA ATG AGG AGA GA3′ 5′ GAA CCA TTC ATG TGC ATG TC 3′ GA WMS182 5′ TGA TGT AGT GAG CCC ATAGGC 3′ 5′ TTG CAC ACA GCC AAA TAA GG 3′ CT WMS186 5′ GCA GAG CCT GGT TCAAAA AG 3′ 5′ CGC CTC TAG CGA GAG CTA TG 5′ GA WMS189 5′ AGG AGC AGC GGAACG AAC 3′ 5′ AGA AAT ACG GAA ACC CAC CC 3′ CA WMS190 5′ GTG CTT GCT GAGCTA TGA GTC 3′ 5′ GTG CCA CGT GGT ACC TTT G 3′ CT,GT WMS191 5′ AGA CTGTTG TTT GCG GGC 3′ 5′ TAG CAC GAC AGT TGT ATG CAT G 3′ CT WMS192 5′ GGTTTT CTT TCA GAT TGC GC 3′ 5′ CGT TGT CTA ATC TTG CCT TGC 3′ CT WMS193 5′CTT TGT GCA CCT CTC TCT CC 3′ 5′ AAT TGT GTT GAT GAT TTG GGG 3′ CT,CAWMS194 5′ GAT CTG CTC TAC TCT CCT CC 3′ 5′ CGA CGC AGA ACT TAA ACA AG 3′CT WMS195 5′ AGG TGC CGT CGC GTC TAC 3′ 5′ ACC CCC CAC GTC AGA GAG 3′ CTWMS197 5′ GAG AAA GAG GTC TGG AGG TCG 3′ 5′ CAA AAT GCA CAA GAA TGG AGG3′ CT WMS198 5′ TTG AAC CGG AAG GAG TAC AG 3′ 5′ TCA GTT TAT TTT GGG CATGTG 3′ CA WMS200 5′ TCA ACG GAA CAG ATG AGC G 3′ 5′ GAC CTG ATG AGA GCAAGC AC 3′ CT WMS203 5′ CCC AAA GCA GCG CAA GC 3′ 5′ ACC AAT GCT ATC GGCTCG 3′ CA,GA WMS205 5′ CGC CCC GGT TCA CTT CAG 3′ 5′ AGT CGC CGT TGT ATAGTG CC 3′ CT WMS210 5′ TGC ATC AAG AAT AGT GTG GAA G 3′ 5′ TGA GAG GAAGGC TCA CAC CT 3′ GA WMS212 5′ AAG CAA CAT TTG CTG CAA TG 3′ 5′ TGC AGTTAA CTT GTT GAA AGG A 3′ CT WMS213 5′ TGC CTG GCT CGT TCT ATC TC 3′ 5′CTA GCT TAG CAC TGT CGC CC 3′ GA WMS218 5′ CGG CAA ACG GAT ATC GAC 3′ 5′AAC AGT AAC TCT CGC CAT AGC C 3′ CT WMS219 5′ GAT GAG CGA CAC CTA GCC TC3′ 5′ GGG GTC CGA GTC CAC AAC 3′ GAimp WMS224 5′ TGA GTC CAG CAC TGC TGC3′ 5′ CAA CAT CCG CTC GTA TTC AA 3′ CT WMS228 5′ TCA TAT GCA CCT CTT TCCTAG G 3′ 5′ GTG TGC CAC CTT TGA CGT C 3′ CT,CA WMS231 5′ AGC TCG GGA TGAAGC GTG 3′ 5′ GAT CCG CCG CTG CGT TT 3′ GAimp WMS232 5′ ATC TCA ACG GCAAGC CG 3′ 5′ CTG ATG CAA GCA ATC CAC C 3′ GA WMS233 5′ TCA AAA CAT AAATGT TCA GA 3′ 5′ TCA ACC GTG TGT AAT TTT GTC C 3′ CT WMS234 5′ GAG TCCTGA TGT GAA GCT GTT G 3′ 5′ CTC ATT GGG GTG TGT ACG TG 3′ CT,CA WMS2375′ GAA TCA CTT GTG AAG CAT CTG G 3′ 5′ CTG GAT GCA TCA CAT CCA AC 3′ CTWMS238 5′ TCG CTT CTA CCG CTC ACC 3′ 5′ AGT GCC TTG CCG AGG TC 3′CT,GT,GGGT WMS241 5′ TCT TCC AAC TAA AGC ATA GC 3′ 5′ CTT CCA TGG ACTACA TAC TAG C 3′ GA WMS242 5′ TCC AAG GCA GTA GGC AGG 3′ 5′ TGT TGT TGGCCT GTA TGC AT 3′ GA WMS244 5′ GGC AGC TGA GGC AAT CTG 3′ 5′ TTT GGA CATTTC CCA GCG 3′ CAimp WMS245 5′ CAG CGC AGT TAG CTC GC 3′ 5′ ATC TGT CCATTC GAG CGC 3′ CT WMS247 5′ GCA ATC TTT TTT CTG ACC ACG 3′ 5′ ATG TGCATG TCG GAC GC 3′ GA WMS248 5′ AGG ACT TCC GCA CCC TG 3′ 5′ TGG CGT GGTCTA AAT GGA C 3′ CA WMS249 5′ CAA ATG GAT CGA GAA AGG GA 3′ 5′ CTG CCATTT TTC TGG ATC TAC C 3′ GAimp WMS251 5′ CAA CTG GTT GCT ACA CAA GCA 3′5′ GGG ATG TCT GTT CCA TCT TAG 3′ CA WMS255 5′ CAA CTG TAC GTA GGT TTCATT GC 3′ 5′ TCT GCC GTA AGT CGC CTC 3′ GA WMS257 5′ AGA GTG CAT GGT GGGACG 3′ 5′ CCA AGA CGA TGC TGA AGT CA 3′ GT WMS258 5′ GAT CGC TTC ATC TCTCTC TCT C 3′ 5′ GTA CAC GCC GTA GGC CC 3′ CT WMS259 5′ AGG GAA AAG ACATCT TTT TTT TC 3′ 5′ CGA CCG ACT TCG GGT TC 3′ GA WMS260 5′ GCC CCC TTGCAC AAA TC 3′ 5′ CGC AGC TAC AGG AGG CC3′ GA WMS261 5′ CTC CCT GTA CGCCTA AGG C 3′ 5′ CTC GCG CTA CTA GCC ATT G 3′ CT WMS263 5′ TCT GCC GTAAGT CGC CTC 3′ 5′ GGT TTC ATT GCT TGC CCT AA 3′ CT WMS264 5′ GAG AAA CATGCC GAA CAA CA 3′ 5′ GCA TGC ATG AGA ATA GGA ACT G 3′ CA WMS265 5′ TGTTGC GGA TGG TCA CTA TT 3′ 5′ GAG TAC ACA TTT GGC CTC TGC 3′ GT WMS268 5′AGG GGA TAT GTT GTC ACT CCA 3′ 5′ TTA TGT GAT TGC GTA CGT ACC C 3′ GAimpWMS269 5′ TGC ATA TAA ACA GTC ACA CAC CC 3′ 5′ TTT GAG CTC CAA AGT GAGTTA GC 3′ CA WMS271 5′ CAA GAT CGT GGA GCC AGC 3′ 5′ AGC TGC TAG CTT TTGGGA CA 3′ CT,GA WMS272 5′ TGC TCT TTG GCG AAT ATA TGG 3′ 5′ GTT CAA AACAAA TTA AAA GGC CC3′ CA WMS273 5′ ATT GGA CGG ACA GAT GCT TT 3′ 5′ AGCAGT GAG GAA GGG GAT C 3′ GA WMS274 5′ AAC TTG CAA AAC TGT TCT GA 3′ 5′TAT TTG AAG CGG TTT GAT TT 3′ OT WMS275 5′ AAT TTT CTT CCT CAC TTA TTC T3′ 5′ AAC AAA AAA TTA GGG CC 3′ CT WMS276 5′ ATT TGC CTG AAG AAA ATA TT3′ 5′ AAT TTC ACT GCA TAC ACA AG 3 CT WMS278 5′ GTT GCT TCA TGA ACG CTCAA 3′ 5′ CTG CCC AAT TTT CTC CAC TC 3′ GTimpGAimp WMS281 5′ CGG CCA TATTTC TGT AAG TAT GC 3 5′ GCA GGT AAT GGC CGG AC 3′ GT WMS282 5′ TTG GCCGTG TAA GGC AG 3′ 5′ TCT CAT TCA CAC ACA ACA CTA GC 3′ GA WMS284 5′ AATGAA AAA ACA CTT GCG TGG 3′ 5′ GCA CAT TTT TCA CTT TCG GG 3′ GA WMS285 5′ATG ACC CTT CTG CCA AAC AC 3′ 5′ ATC GAC CGG GAT CTA GCC 3′ GA WMS291 5′CAT CCC TAC GCC ACT CTG C 3′ 5′ AAT GGT ATC TAT TCC GAC CCG 3′ CA WMS2925′ TCA CCG TGG TCA CCG AC 3′ 5′ CCA CCG AGC CGA TAA TGT AC 3′ CT WMS2935′ TAC TGG TTC ACA TTG GTG CG 3′ 5′ TCG CCA TCA CTC GTT CAA G 3′ CAWMS294 5′ GGA TTG GAG TTA AGA GAG AAC CG 3′ 5′ GCA GAG TGA TCA ATG CCAGA 3′ GAimp WMS295 5′ GTG AAG CAG ACC CAC AAC AC 3′ 5′ GAC GGC TGC GACGTA GAG 3′ GA WMS296 5′ AAT TCA ACC TAC CAA TCT CTG 3′ 5′ GCC TAA TAAACT GAA AAC GAG 3′ CT WMS297 5′ ATC GTC ACG TAT TTT GCA ATG 3′ 5′ TGCGTA AGT CTA GCA TTT TCT G 3′ GT,GA WMS299 5′ ACT ACT TAG GCC TCC CGC C3′ 5′ TGA CCC ACT TGC AAT TCA TC 3′ GA,TAG WMS301 5′ GAG GAG TAA GAC ACATGC CC 3′ 5′ GTG GCT GGA GAT TCA GGT TC 3′ GA,G WMS302 5′ GCA AGA AGCAAC AGC AGT AAC 3′ 5′ CAG ATG CTC TTC TCT GCT GG 3′ GA WMS304 5′ AGG AAACAG AAA TAT CGC GG 3′ 5′ AGG ACT GTG GGG AAT GAA TG 3′ CT WMS311 5′ TCACGT GGA AGA CGC TCC 3′ 5′ CTA CGT GCA CCA CCA TTT TG 3′ GA WMS312 5′ ATCGCA TGA TGC ACG TAG AG 3′ 5′ ACA TGC ATG CCT ACC TAA TGG 3′ GA WMS313 5′CCG CCC TCA TTA AGT TTC AC 3′ 5′ TTT GAC AAG TAC ACG AGT CTG C 3′ CT,GTWMS314 5′ AGG AGC TCC TCT GTG CCA C 3′ 5′ TTC GGG ACT CTC TTC CCT G 3′CT WMS316 5′ CAT GGA CAT TTT ACC ACA AGA C 3′ 5′ TGC GTG TGG TCC ACC TC3′ AT,GT WMS319 5′ GGT TGC TGT ACA AGT GTT CAC G 3′ 5′ CGG GTG CTG TGTGTA ATG AC 3′ CT WMS320 5′ CGA GAT ACT ATG GAA GGT GAG G 3′ 5′ ATC TTTGCA AGG ATT GCC C 3′ GT,GA WMS321 5′ CAA TGT GGA GAC GGT GTG C 3′ 5′ TGTTGC ATG CGA TCA TGC 3′ GT,GAimp WMS322 5′ TCA CAA AAT GAT TTC TCA TCC G3′ 5′ TGC AGA AAA CCA ACA AGG G 3′ GA WMS325 5′ TTT CTT CTG TCG TTC TCTTCC C 3′ 5′ TTT TTA CGC GTC AAC GAC G 3′ CT WMS328 5′ GCA ATC CAC GAGAAG AGA GG 3′ 5′ CAC AAA CTC TTG ACA TGT GCG 3′ GT WMS330 5′ TTG CTA TCCATG TGC CAG AG 3′ 5′ ACA TGT TTC ATG CAG GTA GCC 3′ GTT WMS332 5′ AGCCAG CAA GTC ACC AAA AC 3′ 5′ AGT GCT GGA AAG AGT AGT GAA GC 3′ GA WMS3335′ GCC CGG TCA TGT AAA ACG 3′ 5′ TTT CAG TTT GCG TTA AGC TTT G 3′ GAWMS334 5′ AAT TTC AAA AAG GAG AGA GA 3′ 5′ AAC ATG TGT TTT TAG CTA TC 3′GA WMS335 5′ CGT ACT CCA CTC CAC ACG G 3′ 5′ CGG TCC AAG TGC TAC CTT TC3′ GA,GCGT WMS336 5′ CCC TTT AAT CTC GCT CCC TC 3′ 5′ GTC TCT TTC TCGTAC TTC CAG G 3′ CT WMS337 5′ CCT CTT CCT CCC TCA CTT AGC 3′ 5′ TGC TAACTG GCC TTT GCC 3′ CT,CACT,CA WMS339 5′ AAT TTT CTT CCT CAC TTA TT 3′ 5′AAA CGA ACA ACC ACT CAA TC 3′ CT WMS340 5′ GCA ATC TTT TTT CTG ACC ACG3′ 5′ ACG AGG CAA GAA CAC ACA TG 3′ GA WMS341 5′ TTC AGT GGT AGC GGT CGAG 3′ 5′ CCG ACA TCT CAT GGA TCC AC 3′ CT WMS342 5′ TAT CCA GAG CAG ACGGAC G 3′ 5′ GGT CTA GCT TCG ACG ACA CC 3′ OT WMS344 5′ CAA GGA AAT AGGCGG TAA CT 3′ 5′ ATT TGA GTC TGA AGT TTG CA 3′ GT WMS346 5′ CAA GCA AGGTTT CGT TTT ATC C 3′ 5′ GCA TGT GGT CCA TGT ACT GC 3′ AT,GT WMS349 5′GGC TTC CAG AAA ACA ACA GG 3′ 5′ ATC GGT GCG TAC CAT CCT AC 3′ GA WMS3505′ ACC TCA TCC ACA TGT TCT ACG 3′ 5′ GCA TGG ATA GGA CGC CC 3′ GT WMS3535′ CCA TGT TGA GTA GGT TCA GCC 3′ 5′ CTT GGC CAG AAG CTA CGA AC 3′GCGT,GT WMS356 5′ AGC GTT CTT GGG AAT TAG AGA 3′ 5′ CCA ATC AGC CTG CAACAA C 3′ GA WMS357 5′ TAT GGT CAA AGT TGG ACC TCG 3′ 5′ AGG CTG CAG CTCTTC TTC AG 3′ GA WMS358 5′ AAA CAG CGG ATT TCA TCG AG 3′ 5′ TCC GCT GTTGTT CTG ATC TC 3′ GAimp WMS359 5′ CTA ATT GCA ACA GGT CAT GGG 3′ 5′ TACTTG TGT TCT GGG ACA ATG G 3′ CT,CTTimp WMS361 5′ GTA ACT TGT TGC CAA AGGGG 3′ 5′ ACA AAG TGG CAA AAG GAG ACA 3′ GAimp WMS368 5′ CCA TTT CAC CTAATG CCT GC 3′ 5′ AAT AAA ACC ATG AGC TCA CTT GC 3′ AT WMS369 5′ CTG CAGGCC ATG ATG ATG 3′ 5′ ACC GTG GGT GTT GTG AGC 3′ CTimp WMS371 5′ GAC CAAGAT ATT CAA ACT GGC C 3′ 5′ AGC TCA GCT TGC TTG GTA CC 3′ CA, GA WMS3725′ ATA AGA GCC CTG GGA CTG GG 3′ 5′ GAA GGA CGA CAT TCC ACC TG 3′ GAWMS374 5′ ATA GTG TGT TGC ATG CTG TGT G 3′ 5′ TCT AAT TAG CGT TGG CTG CC3′ GT WMS375 5′ ATT GGC GAC TCT AGC ATA TAC G 3′ 5′ GGG ATG TCT GTT CCATCT TAG C 3′ CA WMS376 5′ GGG CTA GAA AAC AGG AAG GC 3′ 5′ TCT CCC GGAGGG TAG GAG 3′ CA, GAimp WMS382 5′ GTC AGA TAA CGC CGT CCA AT 3′ 5′ CTACGT GCA CCA CCA TTT TG 3′ GA WMS383 5′ ACG CCA GTT GAT CCG TAA AC 3′ 5′GAC ATC AAT AAC CGT GGA TGG 3′ GT WMS384 5′ TTT TCA TGG TGC CCT CTA CT3′ 5′ GCC AAG TTT CTT AGC TAG TTA A 3′ GTimp WMS388 5′ CTA CAA TTC GAAGGA GAG GGG 3′ 5′ CAC CGC GTC AAC TAC TTA AGC 3′ CT,CA,CA WMS389 5′ ATCATG TCG ATC TCC TTG ACG 3′ 5′ TGC CAT GCA CAT TAG CAG AT 3′ CT,GT WMS3905′ AAG TTT CAC ACA AGA TCT CTC C 3′ 5′ TGA CAA GTA CAC GAG TCT GC 3′CT,GT WMS391 5′ ATA GCG AAG TCT CCC TAC TCC A 3′ 5′ ATG TGC ATG TCG GACGC 3′ CA,GA WMS393 5′ TCA TCT GCT ATT TGT GCT ACA 3′ 5′ TCA AAT ACA CCAATG TGC C 3′ CA WMS395 5′ TAC AAC CGC AAG TAA TGC CA 3′ 5′ TAC CAA CACCCT AGC CCT TG 3′ CA WMS397 5′ TGT CAT GGA TTA TTT GGT CGG 3′ 5′ CTG CACTCT CGG TAT ACC AGC 3′ CT WMS400 5′ GTG CTG CCA CCA CTT GC 3′ 5′ TGT AGGCAC TGC TTG GGA G 3′ CA WMS403 5′ CGA CAT TGG CTT CGG TG 3′ 5′ ATA AAACAG TGC GGT CCA GG 3′ CA WMS408 5′ TCG ATT TAT TTG GGC CAC TG 3′ 5′ GTATAA TTC GTT CAC AGC ACG C 3′ CA WMS410 5′ GCT TGA GAC CGG CAC AGT 3′ 5′CGA GAC CTT GAG GGT CTA GA 3′ CA WMS411 5′ CCC ATA CGA TGA TGT GTT TCC3′ 5′ CAA ACG GAA CAT GGT CCC 3′ CT WMS412 5′ ATC AAC AAG GTT TGT GTGTTG G 3′ 5′ ATG AAA CGC GAC CTC CC 3′ GA WMS413 5′ TGC TTG TCT AGA TTGCTT GGG 3′ 5′ GAT CGT CTC GTC CTT GGC A 3′ GA WMS415 5′ GAT CTC CCA TGTCCG CC 3′ 5′ CGA CAG TCG TCA CTT GCC TA 3′ GAimp WMS425 5′ GAG CCC ACAAGC TGG CA 3′ 5′ TCG TTC TCC CAA GGC TTG 3′ CT WMS427 5′ AAA CTT AGA ACTGTA ATT TCA GA 3′ 5′ AGT GTG TTC ATT TGA CAG TT 3′ CA WMS428 5′ CGA GGCAGC GAG GAT TT 3′ 5′ TTC TCC ACT AGC CCC GC 3′ GA WMS429 5′ TTG TAC ATTAAG TTC CCA TTA 3′ 5′TTT AAG GAC CTA CAT GAC AC 3′ CT WMS434 5′ ATG AGTTCC GCC AAA GAA TG 3′ 5′ ACG AAA TAC ACA AGT GGG ACA 3′ GT WMS437 5′ GATCAA GAC TTT TGT ATC TCT C 3′ 5′ GAT GTC CAA CAG TTA GCT TA 3′ CT WMS4405′ CCT ATG GTC TCC ATC ATG AGG 3′ 5′ TCA TGT CAA CTC AAG AAC ACG 3′ CTWMS443 5′ GGG TCT TCA TCC GGA ACT CT 3′ 5′ CCA TGA TTT ATA AAT TCC ACC3′ CA,GA WMS445 5′ TTT GTT GGG GGT TAG GAT TAG 3′ 5′ CCT TAA CAC TTG CTGGTA GTG A 3′ CT WMS448 5′ AAA CCA TAT TGG GAG GAA AGG 3′ 5′ CAC ATG GCATCA CAT TTG TG 3′ GA WMS455 5′ ATT CGG TTC GCT AGC TAC CA 3′ 5′ ACG GAGAGC AAC CTG CC 3′ GTimp WMS456 5′ TCT GAA CAT TAC ACA ACC CTG A 3′ 5′TGC TCT CTC TGA ACC TGA AGC 3′ GA WMS458 5′ AAT GGC AAT TGG AAG ACA TAGC 3′ 5′ TTC GCA ATG TTG ATT TGG C 3′ CA WMS459 5′ ATG GAG TGG TCA CACTTT GAA 3′ 5′ AGC TTC TCT GAC CAA CTT CTC G 3′ GA WMS469 5′ CAA CTC AGTGCT CAC ACA ACG 3′ 5′ CGA TAA CCA CTC ATC CAC ACC 3′ CT WMS471 5′ CGGCCC TAT CAT GGC TG 3′ 5′ GCT TGC AAG TTC CAT TTT GC 3′ CA WMS473 5′ TCATAC GGG TAT GGT TGG AC 3′ 5′ CAC CCC CTT GTT GGT CAC 3′ GTimp WMS476 5′ATG GGT TCG TAC TAA CAT CAG C 3′ 5′ TTG CTG GTA GCT TCA ATC CC 3′ GAimpWMS480 5′ TGC TGC TAC TTG TAC AGA GGA C 3′ 5′ CCG AAT TGT CCG CCA TAG 3′CT, CA WMS484 5′ ACA TCG CTC TTC ACA AAC CC3′ 5′ AGT TCC GGT CAT GGC TAGG 3′ CT WMS494 5′ ATT GAA CAG GAA GAC ATC AGG G 3′ 5′ TTC CTG GAG CTGTCT GGC 3′ CA WMS495 5′ GAG AGC CTC GCG AAA TAT AGG 3′ 5′ TGC TCC TGGTGT TCC TTC G 3′ GA WMS497 5′ GTA GTG AAG ACA AGG GCA TT 3′ 5′ CCG AAAGTT GGG TGA TAT AC 3′ GTimp WMS499 5′ ACT TGT ATG CTC CAT TGA TTG G 3′5′ GGG GAG TGG AAA CTG CAT AA 3′ GA WMS501 5′ GGC TAT CTC TGG CGC TAA AA3′ 5′ TCC ACA AAC AAG TAG CGC C 3′ CA WMS512 5′ AGC CAC CAT CAG CAA AAATT 3′ 5′ GAA CAT GAG CAG TTT GGC AC 3′ GT WMS513 5′ ATC CGT AGC ACC TACTGG TCA 3′ 5′ GGT CTG TTC ATG CCA CAT TG 3′ CA WMS515 5′ AAC ACA ATG GCAAAT GCA GA 3′ 5′ CCT TCC TAG TAA GTG TGC CTC A 3′ GTimp WMS518 5′ AATCAC AAC AAG GCG TGA CA 3′ 5′ CAG GGT GGT GCA TGC AT 3′ CA WMS530 5′ AAATAG GAC AAC CCA CGG C 3′ 5′ TCA ACT TCT TGG CCT CCA TC 3′ CT WMS532 5′ACT GCG TGT GCC TAC AAT TG 3′ 5′ TCA CTC GCA CTC GAT AGG C 3′ GT WMS5335′ AAG GCG AAT CAA ACG GAA TA 3′ 5′ GTT GCT TTA GGG GAA AAG CC 3′ CT,CAWMS537 5′ ACA TAA TGC TCC CTG TGC ACC 3′ 5′ GCC ACT TTT GTG TCG TTC CT3′ CA,TA WMS538 5′ GCA TTT CGG GTG AAC CC 3′ 5′ GTT GCA TGT ATA CGT TAAGCG G 3′ GTimp WMS540 5′ TCT CGC TGT GAA ATC CTA TTT C 3′ 5′ AGG CAT GGATAG AGG GGC 3′ CTimp WMS544 5′ TAG AAT TCT TTA TGG GGT CTG C 3′ 5′ AGGATT CCA ATC CTT CAA AAT T 3′ CT,ATCT,CT WMS550 5′ CCC ACA AGA ACC TTTGAA GA 3′ 5′ CAT TGT GTG TGC AAG GCA C 3′ CT,GT WMS554 5′ TGC CCA CAACGG AAC TTG 3′ 5′ GCA ACC ACC AAG CAC AAA GT 3′ CT,GTimp WMS565 5′ GCGTCA GAT ATG CCT ACC TAG G 3′ 5′ AGT GAG TTA GCC CTG AGC CA 3′ CA WMS5665′ TCT GTC TAC CCA TGG GAT TTG 3′ 5′ CTG GCT TCG AGG TAA GCA AC 3′ CA,TAWMS569 5′ GGA AAC TTA TTG ATT GAA AT 3′ 5′ TCA ATT TTG ACA GAA GAA TT 3′GT WMS570 5′ TCG CCT TTT ACA GTC GGC 3′ 5′ ATG GGT AGC TGA GAG CCA AA 3′CT,GT WMS573 5′ AAG AGA TAA CAT GCA AGA AA 3′ 5′ TTC AAA TAT GTG GGA ACTAC 3′ CA WMS577 5′ ATG GCA TAA TTT GGT GAA ATT G 3′ 5′ TGT TTC AAG CCCAAC TTC TAT T 3′ CA,TA WMS582 5′ AAG CAC TAC GAA AAT ATG AC 3′ 5′ TCTTAA GGG GTG TTA TCA TA 3′ CA WMS583 5′ TTC ACA CCC AAC CAA TAG CA 3′ 5′TCT AGG CAG ACA CAT GCC TG 3′ CA WMS588 5′ GAT CCC CAA TTG CAT GTT G 3′5′ CTT GCA ACT GGG GGA CAC 3′ GT


6. A method for the preparation of a microsatellite marker of claims 1to 5 for plants of the Triticum aestivum species as well of the TribeTriticeae, characterized in that hypervariable genome sections(so-called microsatellites), with the help of the polymerase chainreaction (PCR), are amplified, subsequently separated and detected topolymorphous fragments in the presence of two specific primers, whichflank a microsatellite sequence to the left and right of eachmicrosatellite locus.
 7. The method of claim 6, characterized in thathighly resolving agarose gels, native polyacrylamide gels or denaturingpolyacrylamide gels are used for the separation of the markers.
 8. Themethod of claim 6, characterized in that, depending on the separationsystem, the detection is carried out by means of ethidium bromidestaining, silver staining, radiographic labeling followed byautoradiography or by means of automatic sequencing equipment using dye-or fluorescence-labeled primers.
 9. The use of the microsatellitemarkers of claims 1 to 7, for the genetic analysis of hexaploid andtetraploid cultivated forms of wheat.
 10. The use according to claim 8for the genetic mapping and marking of monogenic and polygenicproperties and their selection for analyzing relationships andidentifying varieties, as well as for evaluating the purity ofvarieties, identifying hybrids and breeding plants.